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Registros recuperados: 115
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Déterminisme du recrutement larvaire de l’huître creuse (Crassostrea gigas) dans la lagune de Thau. ArchiMer
Lagarde, Franck; Fiandrino, Annie; Richard, Marion; Bernard, Ismael; Mortreux, Serge; Ubertini, Martin; Chiantella, Claude; Boj, Marie; Meddah, Slem; Leurion, Axel; Soriano, Solenn; Fuhrmann, Marine; Le Gall, Patrik; Chiera, Caroline; Berteaux, Tom; Guillou, Jean-louis; Roque D'Orbcastel, Emmanuelle; Perignon, Adeline; Bonnet, Delphine; Roques, Cecile; Bec, Beatrice; Cochet, Helene; Gervasoni, Erika; Miron, Gilles; Pouvreau, Stephane.
Since spat mortality events that were firstly detected in 2008, Mediterranean shellfish industry seeks to exploit the Pacific oyster spat from lagoon natural recruitment. The CRCM, Cepralmar and Ifremer developed accordingly a research project named PRONAMED Phase 1 and 2 - 2010-2014, in order to define the economic potential of spatfall and improve knowledge of the reproductive cycle of Crassostrea gigas in this specific environmental context. The results of the past three year‘s study (2012, 2013, 2014) showed that broodstock have the ability to provide larvae after sexual maturation and spawning. A coincidence was noted between moon phase (new and full moon) and spawnings with an amplifying effect of storms on the intensity of spawning (Ubertini et al....
Tipo: Text Palavras-chave: Lagune; Recrutement; Naissain; Huître creuse; Ecologie larvaire; Cycle de reproduction; Captage naturel.; Lagoon; Recruitment; Spat; Pacific oysters; Larval ecology; Reproduction Cycle; Natural spat collection..
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00279/39054/37620.pdf
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DIversification de la pêcherie de la RAde de Brest par l'étude de semis de PEtoncle Noir : le projet DIRAPEN ArchiMer
Pouvreau, Stephane.
Comme de nombreuses espèces d’invertébrés marins natifs de nos côtes, le pétoncle noir (Mimachlamys varia) connaît, depuis 30 ans, une très forte diminution de son abondance avec une disparition progressive des gisements sauvages autrefois réputés. Dans ce contexte, le développement d’une activité de « Sea ranching » peut être une piste intéressante pour soutenir certaines pêcheries côtières, comme c’est le cas pour la coquille Saint Jacques en rade de Brest depuis les années 1990. L’objectif du projet DIRAPEN était donc de lancer à nouveau, en 2015 et sur le site pilote de rade de Brest, des opérations de semis de naissains de pétoncles noirs afin d'étudier les conditions d’un soutien au stock pour cette espèce. Ce rapport présente l’accompagnement...
Tipo: Text Palavras-chave: Pétoncle; Mimachlamys varia; Sea Ranching; Semis; Restauration écologique; Rade de Brest (France); Variegated Scallop; Mimachlamys varia; Sea Ranching; Shellfish mariculture; Ecological restoration; Bay of Brest (France).
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00363/47425/47446.pdf
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Duality of trophic supply and hydrodynamic connectivity drives spatial patterns of Pacific oyster recruitment ArchiMer
Lagarde, Franck; Fiandrino, Annie; Ubertini, Martin; Roque D'Orbcastel, Emmanuelle; Mortreux, Serge; Chiantella, Claude; Bec, Beatrice; Bonnet, Delphine; Roques, Cécile; Bernard, Ismael; Richard, Marion; Guyondet, Thomas; Pouvreau, Stephane; Lett, Christophe.
The recent discovery of Pacific oyster Crassostrea gigas (also known as Magallana gigas) spatfields in a Mediterranean lagoon intensely exploited for shellfish farming (Thau lagoon) revealed significant contrasts in spatial patterns of recruitment. We evaluated the processes that drive spatial patterns in oyster recruitment by comparing observed recruitment, simulated hydrodynamic connectivity and ecological variables. We hypothesized that spatial variability of recruitment depends on (1) hydrodynamic connectivity and (2) the ecology of the larval supply, settlement, metamorphosis, survival and biotic environmental parameters. We assessed recruitment at 6-8 experimental sites by larval sampling and spat collection inside and outside oyster farming areas...
Tipo: Text Palavras-chave: Crassostrea gigas; Coastal lagoon; Larval ecology; Spatial patterns; Connectivity; Settlement; Recruitment; Oligotrophication.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00598/70981/69271.pdf
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Ecophysiological and metabolic adaptations to sulphide exposure of the oyster Crassostrea gigas ArchiMer
Le Moullac, Gilles; Cheize, Marie; Gastineau, Olivier; Daniel, Jean-yves; Le Coz, Jean-rene; Huvet, Arnaud; Moal, Jeanne; Pouvreau, Stephane; Van Wormhoudt, A; Samain, Jean-francois.
The purpose of this study is to examine the response of the oyster Crassostrea gigas to sulphide at different biological scales. A first experiment was designed to measure for 20 h the clearance (CR) and oxygen consumption (OC) rates of oysters exposed at different concentrations of sulphide (0, 3, 6, 12, 20, and 40 mu M). The second experiment was carried out to evaluate the metabolic adaptations to chronic sulphide exposure for 10 days by measuring PK and PEPCK enzyme activities, succinate and alanine content, the adenylate energy charge (AEC), and the activity of the electron transport system (ETS)in whole oysters at above 20 mu M. PK and PEPCK mRNAs and enzyme activities have been measured in adductor muscle. When exposed to sulphide above 20 mu M, CR...
Tipo: Text Palavras-chave: ATP; AEC; Electron transport system; Succcinate; Alanine; Phosphoenolpyruvate carboxykinase; Pyruvate kinase; Oxygen consumption; Clearance rate; Crassostrea gigas; Sulphide; Oyster.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3924.pdf
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Ecophysiological model of growth and reproduction of the black pearl oyster, Pinctada margaritifera: potential applications for pearl farming in French Polynesia ArchiMer
Pouvreau, Stephane; Bacher, Cedric; Heral, Maurice.
A model of bioenergetics of the black pearl oyster (Pinctada margaritifera) was built to simulate growth, reproduction and spawning in suspended culture at field sites in Takapoto lagoon (French Polynesia). This model was based on allometric scaling of physiological functions and scope for growth (SFG) calculations. The input functions were clearance rate (CR, 1 day(-1)), retention efficiency (RE, %) for each kind of particle encountered in suspended matter, pseudofaeces and faeces productions (PF and F, mg C day(-1)), excretion and respiration rate (U and R, mg C day(-1)). The assimilated carbon (i.e., SFG, mg C day(-1)) was partitioned to the three internal state variables (somatic tissue, shell and gonad) according to the asymptotic increase of the...
Tipo: Text Palavras-chave: Pearl farming; Food depletion; Scope for growth; Carrying capacity; Suspension feeding; Tropical lagoon.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-380.pdf
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Ecophysiologie de la reproduction chez l'huître creuse, Crassostrea gigas ArchiMer
Pouvreau, Stephane; Lepennec, M.
The reproductive cycle of Megathura crenulata was documented and quantification of oocyte lipid reserves with lipo-specific stains was explored in both this species and in Crassostrea gigas. The necessity of providing food to female broodstock C. gigas in spring was re-evaluated, because subsequent larval and post-larval rearing performance was not affected by broodstock starvation during conditioning. Developmental study of the organs responsible of particle processing in C. gigas allowed the identification of critical stages related to the ontogenesis of each organ, as well as a better understanding of the evolution of these organs, highly-modified in the Ostreidae. Ciliary type identification and mucocyte mapping from post-larval to adult stage showed...
Tipo: Text Palavras-chave: Mucocytes; Mantle; Gill; Larvae postlarvae juveniles; Feeding; Crassostrea gigas; Megathura crenulata; Development; Reproduction; Mucocytes; Manteau; Branchie; Larves post larves juvéniles; Alimentation; Crassostrea gigas; Megathura crenulata; Développement; Reproduction.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/rapport-1867.pdf
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Effect of environmental and nutritive conditionings on defense mechanisms of oyster Crassostrea gigas during an annual cycle ArchiMer
Delaporte, Maryse; Moal, Jeanne; Soudant, Philippe; Lambert, Christophe; Pouvreau, Stephane; Enriquez, Martha; Degremont, Lionel; Soletchnik, Patrick; Gagnaire, Beatrice; Ropert, Michel.
In the frame of MOREST project, a common biological material, resulting of a mixture of different families produced in experimental hatchery, was reared in two different environmental fields : Normandy and Charente. Concomitantly, a pool was conditioned at the Ifremer Argenton hatchery with three different algae levels : 4%, 8% and 12% of algal dry weight per oyster dry weight. During the experiments, five immune parameters were studied in parallel with survival rate and reproductive status (stages and intensity). Site location, seasonal variations and experimental diet level clearly influenced oyster immune parameters. Haemocyte counts were higher for oysters reared in Normandy than those reared in Charente and in hatchery. Granulocyte percentage was...
Tipo: Text Palavras-chave: Defense mechanisms; Nutrition; Seasonal variations; Physiology; Crassostrea gigas; Pacific oysters.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3500.pdf
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Effect of environmental drivers on the reproduction of the pacific oyster Crassostrea gigas in the Mediterranean: The case of the Thau lagoon ArchiMer
Ubertini, Martin; Lagarde, Franck; Le Gall, Patrik; Mortreux, Serge; Pernet, Fabrice; Fiandrino, Annie; Pouvreau, Stephane; Roque D'Orbcastel, Emmanuelle.
The aquaculture of the oyster Crassostrea gigas is largely based on natural recruitment of the species on spat collectors along the french Atlantic coast, the remaining spat being provided by hatcheries and nurseries. Due to an increase in the climate/meteorological variability, during the last twenty years, the natural spat collection has become variable, from null to overabundant depending on the year. Since 2008, the oyster spat mortalities worsen the situation, reducing the available spat and increasing the cost of the latter. However, the temporal variability of the natural recruitment modalities in the Mediterranean do not allow the aquaculture industry to rely on native spats, making the understanding of natural recruitment in the Mediterranean a...
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00248/35894/34422.pdf
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Effect of phytoplankton and temperature on the reproduction of the Pacific oyster Crassostrea gigas: investigation through DEB theory ArchiMer
Bernard, Ismael; De Kermoysan, Goulwen; Pouvreau, Stephane.
DEB theory can be used to obtain a detailed description of energy allocation in organisms and the control of this allocation by temperature and food concentration. In this study, we modified the model of Bourlès et al. (2009) developed for the Pacific oyster, Crassostrea gigas, to improve the description of reproductive effort. The model was amended in two ways: a new set of parameters was incorporated and a full description of gonad construction in spring was added, with a new state variable. The application of this model to a dataset for oyster growth measured in four bays over two years showed that this model better described reproductive effort, without modifying overall growth dynamics. We then explored the reproductive responses of oysters to their...
Tipo: Text Palavras-chave: DEB model; Crassostrea gigas; Gametogenesis; Reproductive effort; Spawning date; Functional response.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00059/17047/14606.pdf
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Estimation de la biomasse d'huîtres non cultivées dans le bassin de Marennes-Oléron ArchiMer
Prou, Jean; Pouvreau, Stephane; Renaud, Vivian.
In the Marennes-Oléron oyster culture basin, the cultivated oyster stock shows an unbalanced age distribution: 25 000 to 30 000 tonnes are commercialized each year for a total cultivated biomass of 95 000 tonnes (1993). The biological overexploitation of the basin requires a reduction of the stock in order to get back to productivity rates that are compatible with the restrictions of the oyster market. This study gives an estimated natural deposit biomass of around 3 000 tonnes. An estimate of the abandoned and wild oyster biomass shows that 20 000 tonnes of these animals can be considered as competitors to cultivated oysters. [...].
Tipo: Text Palavras-chave: Bassin Marennes Oléron; Echantillonnage; Estimation; Répartition spatiale; Typologie; Biomasse; Crassostrea gigas; Huîtres.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/rapport-4741.PDF
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Estimation, Préservation, Reconstitution des bancs sauvages d’huîtres plates en Baie de Quiberon ArchiMer
Cochet, Hélène; Pouvreau, Stephane; Tanguy, Ludovic; Kerisit, Christophe.
L’huître plate, Ostrea edulis, est l’huître native des côtes européennes. Espèce emblématique de Bretagne, elle a subi deux épizooties successives dans les années 70 qui ont fait chuter sa production de 20 000 tonnes à moins de 2000 tonnes actuellement et disparaître de nombreux bancs naturels. Désormais l’espèce ne se reproduit plus que dans quelques baies et rias situées principalement en Bretagne et Normandie et le captage ne se pratique qu’en Baie de Quiberon et en Rade de Brest. Mais ce dernier est très variable d’une année à l’autre et a été particulièrement faible en Baie de Quiberon depuis 2010. De récentes études ont conclu que la diminution des stocks d’huîtres, notamment des bancs naturels de l’Ouest de la baie, pouvait être l’une des raisons à...
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00304/41488/40700.pdf
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Etude de faisabilité d’opérations de ‘repeuplement orienté’ dans deux sites français de captage de l’huître creuse (Crassostrea gigas) ArchiMer
Bernard, Ismael; Lapegue, Sylvie; Dumas, Franck; Talarmain, Eric; Cornette, Florence; Degremont, Lionel; Chavanne, Herve; Pouvreau, Stephane; Auby, Isabelle; Maurer, Daniele; Plus, Martin; Le Moine, Olivier; Boudry, Pierre.
La forte augmentation des mortalités de juvéniles d’huître creuse depuis 2008 a relancé la problématique de l’approvisionnement en naissains de l’ostréiculture française. Dans ce contexte, cette étude a pour objectif d’étudier la faisabilité d’opérations de repeuplement en huîtres génétiquement plus résistantes, afin d’améliorer les performances de survie du naissain capté dans le milieu naturel. Cette étude s’est ainsi focalisée sur les deux bassins naisseurs historiques, Marennes-Oléron et Arcachon. Nous avons donc cherché à établir (1) une hiérarchie des bancs en fonction de l’expulsion de larves d’huître dans le bassin d’Arcachon, (2) une cartographie des liens en termes de connectivité entre les bancs des pertuis charentais et (3) la faisabilité du...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00083/19410/17013.pdf
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Etude de la connectivité potentielle en rade de Brest : application au cas de l'huître plate, Ostrea edulis ArchiMer
Bernard, Ismael; Duchene, Julie; Hily, Christian; Lazure, Pascal; Pouvreau, Stephane; Boudry, Pierre.
En complément du rapport final (Anonyme (2014). PERLE. Programme d'expérimentation et de recherche sur l'huître plate Ostrea edulis. Rapport final de l'ensemble du projet 2011-2014. http://archimer.ifremer.fr/doc/00248/35932/), ce rapport présente l'étude de la phase de dispersion des larves en rade de Brest et analyse le lien entre la quantité de larves émises et la quantité de larves captées. Ce lien, qui possède une forte composante spatiale, est appelé connectivité. Il est sous l’influence de la dispersion et de la survie des larves mais seule la dispersion larvaire, avec différents forçages biophysiques du milieu, a été considérée ici. Les effets de la localisation des zones d’émission et de captage, du comportement des larves, du vent ou de la durée...
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00251/36233/34793.pdf
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Etude de la fécondité de l’huître creuse dans le bassin d’Arcachon en 2013 en lien avec la qualité de l’eau. Compte-rendu final du programme FeLiBA ArchiMer
Bernard, Ismael; Allain, Gwenhael; Auby, Isabelle; Plus, Martin; Drean, Tugdual; Gasmi, Sonia; David, Valérie; Pouvreau, Stephane; Maurer, Daniele.
De 2009 à 2011, le captage à Arcachon fut particulièrement mauvais. Des pontes tardives associées à de faibles quantités de larves “petites” indiquaient un problème probablement au niveau de la maturation ou de la ponte. Le programme FéLiBA consiste en un zoom, pour l’année 2013, sur la Fécondité des huîtres creuses en Lien avec la qualité de l’eau du Bassin d’Arcachon. Ce programme s’est traduit par différentes actions de terrain. Tout d’abord, la maturation des huîtres issues du captage d’Arcachon ou de Marennes-Oléron a été suivie à Arcachon (site du Tès) et comparée avec celle de la Rade de Brest (pointe du Château) au cours de l’année 2013. Puis, un bilan de la maturation des huîtres sauvages d’Arcachon a été établi fin juin 2013. La ponte a également...
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00179/29002/27428.pdf
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Etude et modélisation des mécanismes impliqués dans la croissance de l'huître perlière, Pinctada margaritifera, au sein de l'écosystème conchylicole du lagon de l'atoll de Takapoto (Polynésie Française) ArchiMer
Pouvreau, Stephane.
L'huître perlière, Pinctada margaritifera (Linné, 1758) var. cumingi (Reeve), productrice de perles noires, fait l' objet d'une aquaculture florissante en Polynésie Française. Afin de pérenniser cette activité, un programme de recherche (le PGRN) a été lancé, en 1990, sur l' atoll de Takapoto. L' un des thèmes de ce programme réside dans l'étude de la capacité trophique d'un lagon soumis à une activité de perliculture. A cet égard, un modèle déterministe expliquant la croissance et la reproduction de P. margaritifera à partir des paramètres du milieu (notamment la nourriture disponible) a été mis au point. La construction de ce modèle a impliqué : ( 1) l'analyse de l'hydrobiologie du lagon; (2) l'étude de la physiologie de P. margaritifera; (3) le sui vi...
Tipo: Text Palavras-chave: Bivalves; Huîtres perlières; Pinctada margaritifera; Physiologie; Croissance; Reproduction; Hydrobiologie; Atoll; Lagon; Écosystème; Réseau trophique; Modélisation; Capacité trophique; Polynésie française; Bivalves; Pearl oyster; Pinctada ma rgaritifera; Physiology; Growth; Reproduction; Hydrobiology; Atoll; Lagoon; Ecosystem; Trophic web; Modelling; Carrying capacity; French Polynesia.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00034/14558/11856.pdf
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Évaluation des potentialités de l'IRM pour la recherche sur la Physiologie des Bivalves Premiers résultats anatomiques et perspectives ArchiMer
Pouvreau, Stephane; Davenel, A; Quellec, S; Rambeau, M.
Nuclear Magnetic Resonance (NMR) is increasingly used in biology and the appearance of imaging and structural and/or metabolic spectroscopy (MRI/MSR) platforms (Rennes, Strasbourg, Marseille...) devoted to this small animal shows the recent keen interest in these research techniques. However, to this day and to our knowledge, no approach of this style has been developed for sea molluscs and even less so for the cup oyster, Crassostrea gigas. Because of its economic importance, this bivalve mollusc is the subject of much research in physiology, to the point of becoming, at IFREMER, a biological model for marine invertebrates. Nonetheless, most of the methodologies developed for studying it are destructive, which (1) prevents individual follow-up, (2)...
Tipo: Text Palavras-chave: Nuclear Magnetic Resonance; Anatomy; Biometry; Physiology; Bivalvia.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/rapport-1560.pdf
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Experimental shift in diet Delta13C: A potential tool for ecophysiological studies in marine bivalves ArchiMer
Paulet, Yves-marie; Lorrain, Anne; Richard, Joëlle; Pouvreau, Stephane.
To test the potential of diet switching experiments in ecophysiological studies of marine invertebrates, stable carbon isotope ratios were measured at different seasons in the gonad, adductor muscle, digestive gland and gills of scallops (Pecten maximus) and oysters (Crassostrea gigas) held for 15 days on a constant diet of phytoplankton depleted in 13C. The aim of this study was to determine if differences in carbon incorporation could be detected among species, seasons and organs, and if so, whether it was consistent with their known energy-allocation patterns. After offering the new diet, isotope values of the different organs gradually shifted and significant differences among organs, seasons and species were found. A carbon incorporation index (CII)...
Tipo: Text Palavras-chave: Crassostrea gigas; Pecten maximus; Carbon isotopes; Metabolism; Energy allocation.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1202.pdf
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Forty questions of importance to the policy and practice of native oyster reef restoration in Europe ArchiMer
Zu Ermgassen, Philine S. E.; Bonačić, Kruno; Boudry, Pierre; Bromley, Cass A.; Cameron, Tom C.; Colsoul, Bérenger; Coolen, Joop W. P.; Frankić, Anamarija; Hancock, Boze; Have, Tom M.; Holbrook, Zoë; Kamermans, Pauline; Laugen, Ane T.; Nevejan, Nancy; Pogoda, Bernadette; Pouvreau, Stephane; Preston, Joanne; Ranger, Christopher J.; Sanderson, William G.; Sas, Hein; Strand, Åsa; Sutherland, William J..
Oyster reefs are among the most threatened marine habitats globally. In Europe, oyster reefs have been extirpated from most locations within their historical range. Active restoration of the native oyster (Ostrea edulis) in Europe has grown substantially in recent years. In sharing experiences between oyster restoration projects in Europe at the Native Oyster Restoration Alliance conference, NORA2, in Edinburgh in May 2019, it became apparent that a number of similar barriers are experienced. This study identified the top 40 questions, which, if answered, would have the greatest influence on the policy and practice of oyster restoration in Europe. Initially 71 people were consulted across 28 institutions and 11 European countries to generate 194 questions....
Tipo: Text Palavras-chave: Alien species; Estuary; Fishing; Invertebrates; Pollution; Restoration; Subtidal.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00658/77004/78261.pdf
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Gametogenesis, reproductive investment, and spawning behavior of the Pacific giant oyster Crassostrea gigas: evidence of an environment-dependent strategy ArchiMer
Enriquez-diaz, M.; Pouvreau, Stephane; Chavez-villalba, J.; Le Pennec, M..
The progress of gametogenesis was studied in oysters Crassostrea gigas having the same origin (Tremblade), but cultured during 1 year in two distinctive French marine areas, the Baie des Veys and Marennes-Ol,ron. We assessed seasonal changes in the reproduction cycle on the basis of stereological techniques to estimate reproductive investment and measurement of gonad evolution area by quantitative histology. From a qualitative point of view, both oyster groups presented typical reproductive stages, but showed differential timing, in particular during the sequence of spawning and duration of the re-absorption stage. Oysters in Baie des Veys had a single partial spawning in August and a re-absorption stage that extended until winter. Oysters in...
Tipo: Text Palavras-chave: Stereology; Reproductive investment; Pacific oysters; Image analysis; Gametogenesis; Crassostrea gigas.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7300.pdf
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Gametogenesis, spawning behavior and larval abundance of the Pacific oyster Crassostrea gigas in the Thau lagoon: Evidence of an environment-dependent strategy ArchiMer
Ubertini, Martin; Lagarde, Franck; Mortreux, Serge; Le Gall, Patrik; Chiantella, Claude; Fiandrino, Annie; Bernard, Ismael; Pouvreau, Stephane; Roque D'Orbcastel, Emmanuelle.
The Thau lagoon, located at the South of France, provides 10% of the French pacific oyster Crassostrea gigas production. Despite this intensive shellfish production, the larval recruitment of this species within the Thau lagoon is still misunderstood and spat collection remains highly variable; as a consequence, shellfish farmers rely mostly on hatchery”s spat. The present study aimed to describe reproduction features of C. gigas within the Mediterranean, as well as to explore spawning triggers of this species. To achieve this goal, the reproduction cycle from gametogenesis to spawning was followed during two years in several locations within the Mediterranean Thau lagoon. The spawning behavior of C. gigas within the Thau lagoon appeared to be slightly...
Tipo: Text Palavras-chave: Mollusc physiology; Spawning triggers; Oysters.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00369/48032/48118.pdf
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